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Enzyme Inhibition Calculator – Competitive Inhibition | FreeCalz Enzyme Inhibition Calculator Calculate enzyme reaction velocity with competitive inhibition using Vmax, Km, substrate concentration [S], inhibitor concentration [I], and inhibition constant Ki. Estimate inhibited velocity, apparent Km, and the inhibition factor. Competitive Enzyme Inhibition Calculator Enter the kinetic parameters and concentrations below to calculate reaction velocity … Read more
Enzyme Kinetics Calculator – Michaelis-Menten Equation | FreeCalz Enzyme Kinetics Calculator Calculate enzyme reaction velocity using the Michaelis-Menten equation from maximum velocity (Vmax), Michaelis constant (Km), and substrate concentration [S]. Explore reaction rate, substrate saturation, and the relationship between velocity and Vmax. Michaelis-Menten Enzyme Kinetics Calculator Enter Vmax, Km, and substrate concentration [S]. The calculator … Read more
Protein Concentration Calculator – mg/mL, A280 & Standard Curve | FreeCalz Protein Concentration Calculator Calculate protein concentration from mass and solution volume, UV absorbance at 280 nm, or a protein-assay standard curve. View results in mg/mL and g/L, with optional molar concentration when molecular weight is available. Protein Concentration Calculator Choose the calculation method that … Read more
Beer-Lambert Law Calculator – Absorbance & Concentration | FreeCalz Beer-Lambert Law Calculator Calculate molar concentration from absorbance using the Beer-Lambert law. Enter absorbance, molar absorption coefficient and optical path length to determine concentration in mol/L, mM and, when molecular weight is supplied, mg/mL. Beer-Lambert Law Calculator Enter the measured absorbance, molar absorption coefficient (ε), and … Read more
qPCR ΔΔCt Calculator – Relative Gene Expression | FreeCalz qPCR ΔΔCt Calculator Calculate relative gene expression using the comparative Ct (ΔΔCt) method. Enter target-gene and reference-gene Ct values for control and treated samples to calculate ΔCt, ΔΔCt, relative fold change and percentage expression. qPCR ΔΔCt Calculator Enter four Ct values: target and reference genes for … Read more
PCR Annealing Temperature Calculator – PCR Ta | FreeCalz PCR Annealing Temperature Calculator Calculate a starting PCR annealing temperature (Ta) from the melting temperatures (Tm) of your forward and reverse primers. Compare common PCR rules, estimate a useful gradient range, and understand why polymerase-specific protocols can require different settings. PCR Annealing Temperature Calculator Enter the … Read more
PCR Product Size Calculator – Amplicon Length | FreeCalz PCR Product Size Calculator Calculate the expected PCR product or amplicon length from forward and reverse primer positions. Enter the primer coordinates using a clear coordinate convention and obtain the expected product size with a step-by-step calculation. PCR Product Size Calculator Use primer coordinates on a … Read more
PCR Efficiency Calculator – qPCR Standard Curve | FreeCalz PCR Efficiency Calculator Calculate PCR amplification efficiency from the slope of a qPCR standard curve. Enter the standard-curve slope to calculate efficiency, amplification factor and key interpretation values for assay evaluation. PCR Efficiency Calculator Enter the slope from a standard curve where Cq/Ct is plotted against … Read more
PCR Master Mix Calculator – Reaction Setup & Volumes | FreeCalz PCR Master Mix Calculator Calculate PCR master mix volumes for multiple reactions. Enter the reaction volume, master mix concentration, primer stocks, template volume and extra-volume allowance to determine per-reaction and total volumes for a practical PCR setup. PCR Master Mix Calculator This calculator is … Read more
DNA to RNA Converter – Transcription Sequence Calculator | FreeCalz DNA → RNA Converter Convert a DNA sequence into its corresponding RNA sequence using coding- or template-strand transcription rules. Choose the strand type and input orientation, then review the RNA sequence, base statistics and step-by-step conversion. DNA → RNA Converter Enter a DNA sequence, identify … Read more